thp 1 cd14 blue cells Search Results


thp 1 md2 cd14 cells  (ATCC)
99
ATCC thp 1 md2 cd14 cells
Thp 1 Md2 Cd14 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thp+1+cd14+blue+cells/THP-1/pm34804025-49-16-51
Average 99 stars, based on 1 article reviews
thp 1 md2 cd14 cells - by Bioz Stars, 2026-09
99/100 stars
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thp1-dual md2-cd14-tlr4 cells  (InvivoGen)
N/A
TLR4, MD-2 and CD14 expressing NF-κB and IRF reporter monocytes
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thp1-fcγriia-r131 + -cd14 + cell lines  (Novimmune)
90
Novimmune thp1-fcγriia-r131 + -cd14 + cell lines
Differential regulation of FcR-ITAM signals by Lyn and Fyn. a After induction of <t>FcγRIIA-ITAMi</t> or ITAM signalling in <t>THP-1-CD14</t> + -FcγRIIA + cells transfected with indicated siRNAs, immunoprecipitation (IP) and immunoblots (IB) were performed with indicated Abs. Quantification of the indicated band using ImageJ software relative to total corresponding protein levels in cell lysates (see Supplementary Fig. ) is indicated at the bottom of each panel, representing one out of at least three experiments. b Modulation of LPS-mediated IL-8 production by Lyn and Fyn during FcγRIIA-ITAMi induction. THP-1-CD14 + -FcγRIIA + cells transfected with indicated siRNAs were stimulated for indicated time points to induce either ITAMi or ITAM signals followed by stimulation with LPS (10 ng/ml) for 1 h. Then, supernatant was collected for cytokine measurement. c Modulation of IL-8 production by Lyn and Fyn during FcγRIIA-ITAM induction for 18 h. Data are presented as the mean ± s.e.m. *** P < .001; Student’s unpaired t- test
Thp1 Fcγriia R131 + Cd14 + Cell Lines, supplied by Novimmune, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thp+1+cd14+blue+cells/thp+1+fc%CE%B3riia+r131++++cd14+++cell+lines/pmc05557797-237-6-15
Average 90 stars, based on 1 article reviews
thp1-fcγriia-r131 + -cd14 + cell lines - by Bioz Stars, 2026-09
90/100 stars
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thp-1blue (cd14 lo  (Autogen-Bioclear ltd)
90
Autogen-Bioclear ltd thp-1blue (cd14 lo
<t>THP-1-derived</t> M1 and M2 MΦs were generated by differentiating THP-1 monocytes with either 25 ng/ml phorbol 12-myristate 13-acetate (PMA) for 3 days or 10 nM 1,25-(OH) 2 vitamin D 3 for 7 days, respectively. M1 (bold) and M2 (shaded) MΦ subsets were stimulated with either 100 ng/ml PG-LPS (a, b and c) or 1×10 7 cells/ml HKPG (d, e and f). Cytokine production is expressed as the mean ± SD in pg/ml for TNFα (a & d), IL-1β (b & e) and IL-6 (c & f). Data displayed represents triplicate samples for n = 3 replicate experiments. Significant differences in cytokine production between activated M1 and M2 MΦs are indicated as *p<0.05, **p<0.01, ***P<0.001 and ns, not significant.
Thp 1blue (Cd14 Lo, supplied by Autogen-Bioclear ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thp+1+cd14+blue+cells/mouse+monoclonal+anti+cd14++sh+m1++antibody/pmc03711904-75-1-15
Average 90 stars, based on 1 article reviews
thp-1blue (cd14 lo - by Bioz Stars, 2026-09
90/100 stars
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monocytes (thp-1)  (CEM Corporation)
90
CEM Corporation monocytes (thp-1)
<t>THP-1-derived</t> M1 and M2 MΦs were generated by differentiating THP-1 monocytes with either 25 ng/ml phorbol 12-myristate 13-acetate (PMA) for 3 days or 10 nM 1,25-(OH) 2 vitamin D 3 for 7 days, respectively. M1 (bold) and M2 (shaded) MΦ subsets were stimulated with either 100 ng/ml PG-LPS (a, b and c) or 1×10 7 cells/ml HKPG (d, e and f). Cytokine production is expressed as the mean ± SD in pg/ml for TNFα (a & d), IL-1β (b & e) and IL-6 (c & f). Data displayed represents triplicate samples for n = 3 replicate experiments. Significant differences in cytokine production between activated M1 and M2 MΦs are indicated as *p<0.05, **p<0.01, ***P<0.001 and ns, not significant.
Monocytes (Thp 1), supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thp+1+cd14+blue+cells/thp+1+cells/10__1007_slash_s13546___011___0353___9-22-2-51
Average 90 stars, based on 1 article reviews
monocytes (thp-1) - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Differential regulation of FcR-ITAM signals by Lyn and Fyn. a After induction of FcγRIIA-ITAMi or ITAM signalling in THP-1-CD14 + -FcγRIIA + cells transfected with indicated siRNAs, immunoprecipitation (IP) and immunoblots (IB) were performed with indicated Abs. Quantification of the indicated band using ImageJ software relative to total corresponding protein levels in cell lysates (see Supplementary Fig. ) is indicated at the bottom of each panel, representing one out of at least three experiments. b Modulation of LPS-mediated IL-8 production by Lyn and Fyn during FcγRIIA-ITAMi induction. THP-1-CD14 + -FcγRIIA + cells transfected with indicated siRNAs were stimulated for indicated time points to induce either ITAMi or ITAM signals followed by stimulation with LPS (10 ng/ml) for 1 h. Then, supernatant was collected for cytokine measurement. c Modulation of IL-8 production by Lyn and Fyn during FcγRIIA-ITAM induction for 18 h. Data are presented as the mean ± s.e.m. *** P < .001; Student’s unpaired t- test

Journal: Nature Communications

Article Title: Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation

doi: 10.1038/s41467-017-00294-0

Figure Lengend Snippet: Differential regulation of FcR-ITAM signals by Lyn and Fyn. a After induction of FcγRIIA-ITAMi or ITAM signalling in THP-1-CD14 + -FcγRIIA + cells transfected with indicated siRNAs, immunoprecipitation (IP) and immunoblots (IB) were performed with indicated Abs. Quantification of the indicated band using ImageJ software relative to total corresponding protein levels in cell lysates (see Supplementary Fig. ) is indicated at the bottom of each panel, representing one out of at least three experiments. b Modulation of LPS-mediated IL-8 production by Lyn and Fyn during FcγRIIA-ITAMi induction. THP-1-CD14 + -FcγRIIA + cells transfected with indicated siRNAs were stimulated for indicated time points to induce either ITAMi or ITAM signals followed by stimulation with LPS (10 ng/ml) for 1 h. Then, supernatant was collected for cytokine measurement. c Modulation of IL-8 production by Lyn and Fyn during FcγRIIA-ITAM induction for 18 h. Data are presented as the mean ± s.e.m. *** P < .001; Student’s unpaired t- test

Article Snippet: THP-1 (ATCC, catalog # TIB-202) and THP1-FcγRIIA-R131 + -CD14 + cell lines (kindly provided by Novimmune) were maintained in RPMI-1640, 10% FCS and 50 μM β-mercaptoethanol or supplemented with 200 μg/ml Zeocin, 10 μg/ml blasticidin and 2 μg/ml puromycin (Invitrogen, France).

Techniques: Transfection, Immunoprecipitation, Western Blot, Software

THP-1-derived M1 and M2 MΦs were generated by differentiating THP-1 monocytes with either 25 ng/ml phorbol 12-myristate 13-acetate (PMA) for 3 days or 10 nM 1,25-(OH) 2 vitamin D 3 for 7 days, respectively. M1 (bold) and M2 (shaded) MΦ subsets were stimulated with either 100 ng/ml PG-LPS (a, b and c) or 1×10 7 cells/ml HKPG (d, e and f). Cytokine production is expressed as the mean ± SD in pg/ml for TNFα (a & d), IL-1β (b & e) and IL-6 (c & f). Data displayed represents triplicate samples for n = 3 replicate experiments. Significant differences in cytokine production between activated M1 and M2 MΦs are indicated as *p<0.05, **p<0.01, ***P<0.001 and ns, not significant.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: THP-1-derived M1 and M2 MΦs were generated by differentiating THP-1 monocytes with either 25 ng/ml phorbol 12-myristate 13-acetate (PMA) for 3 days or 10 nM 1,25-(OH) 2 vitamin D 3 for 7 days, respectively. M1 (bold) and M2 (shaded) MΦ subsets were stimulated with either 100 ng/ml PG-LPS (a, b and c) or 1×10 7 cells/ml HKPG (d, e and f). Cytokine production is expressed as the mean ± SD in pg/ml for TNFα (a & d), IL-1β (b & e) and IL-6 (c & f). Data displayed represents triplicate samples for n = 3 replicate experiments. Significant differences in cytokine production between activated M1 and M2 MΦs are indicated as *p<0.05, **p<0.01, ***P<0.001 and ns, not significant.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques: Derivative Assay, Generated

THP-1-derived CD14-high- and CD14-low-expressing (CD14 hi and CD14 lo ) M1 and M2 MΦs were generated by differentiating CD14 + and CD14 − stable transfectant THP-1-blue monocytes with either 25 ng/ml phorbol 12-myristate 13-acetate (PMA) for 3 days or 10 nM 1,25-(OH) 2 vitamin D 3 for 7 days, respectively. CD14 hi /CD14 lo M1 (bold) and M2 (shaded) MΦ subsets were stimulated with either 100ng/ml PG-LPS (a, b, c & d) or 1×10 7 cells/ml HKPG (e, f, g & h). Cytokine production is expressed as the mean ± SD in pg/ml for TNFα (a & e), IL-1β (b & f), IL-6 (c & g) and IL-10 (d & h). Data displayed represents triplicate samples for n = 3 replicate experiments. Significant differences in cytokine production between activated CD14 hi and CD14 lo MΦs are indicated as *p<0.05, **p<0.01, ***P<0.001 and ns, not significant.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: THP-1-derived CD14-high- and CD14-low-expressing (CD14 hi and CD14 lo ) M1 and M2 MΦs were generated by differentiating CD14 + and CD14 − stable transfectant THP-1-blue monocytes with either 25 ng/ml phorbol 12-myristate 13-acetate (PMA) for 3 days or 10 nM 1,25-(OH) 2 vitamin D 3 for 7 days, respectively. CD14 hi /CD14 lo M1 (bold) and M2 (shaded) MΦ subsets were stimulated with either 100ng/ml PG-LPS (a, b, c & d) or 1×10 7 cells/ml HKPG (e, f, g & h). Cytokine production is expressed as the mean ± SD in pg/ml for TNFα (a & e), IL-1β (b & f), IL-6 (c & g) and IL-10 (d & h). Data displayed represents triplicate samples for n = 3 replicate experiments. Significant differences in cytokine production between activated CD14 hi and CD14 lo MΦs are indicated as *p<0.05, **p<0.01, ***P<0.001 and ns, not significant.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques: Derivative Assay, Expressing, Generated, Transfection

PG-LPS exhibits weak endotoxin activity in CD14 hi/lo M1/M2 macrophages.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: PG-LPS exhibits weak endotoxin activity in CD14 hi/lo M1/M2 macrophages.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques: Activity Assay

CD14 lo (bold) and CD14 hi (shaded) M1 and M2 MΦ subsets were stimulated with either 100 ng/ml PG-LPS or 1×10 7 cells/ml HKPG. NFκB activation is expressed as the mean absorbance units A 620nm ± SD for M1 (a) and M2 (b) MΦ subsets. Data displayed represents triplicate samples for n = 3 replicate experiments. Significant differences in NFκB activation between CD14 hi and CD14 lo MΦs are indicated as *p<0.05, **p<0.01 and ns, not significant.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: CD14 lo (bold) and CD14 hi (shaded) M1 and M2 MΦ subsets were stimulated with either 100 ng/ml PG-LPS or 1×10 7 cells/ml HKPG. NFκB activation is expressed as the mean absorbance units A 620nm ± SD for M1 (a) and M2 (b) MΦ subsets. Data displayed represents triplicate samples for n = 3 replicate experiments. Significant differences in NFκB activation between CD14 hi and CD14 lo MΦs are indicated as *p<0.05, **p<0.01 and ns, not significant.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques: Activation Assay

CD14 lo M1 (a) CD14 hi M1 (b), CD14 lo M2 (c) and CD14 hi M2 (d) MΦ subsets were pre-stimulated (tolerised) with either 100 ng/ml PG-LPS (unshaded) or 1×10 7 cells/ml HKPG (shaded) for 24 hours prior to stimulation with PG-LPS or HKPG and incubated for a further 18 hours (untolerised controls indicated in bold). NFκB activation is expressed as the mean absorbance units A 620nm ± SD for the CD14 hi/lo M1 and M2 MΦ subsets. Data displayed represents triplicate samples for n = 3 replicate experiments. Significant effects compared to the un-tolerised stimulus control (bold) for each MΦ subset are indicated as *p<0.05, ***p<0.001 and ns, not significant.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: CD14 lo M1 (a) CD14 hi M1 (b), CD14 lo M2 (c) and CD14 hi M2 (d) MΦ subsets were pre-stimulated (tolerised) with either 100 ng/ml PG-LPS (unshaded) or 1×10 7 cells/ml HKPG (shaded) for 24 hours prior to stimulation with PG-LPS or HKPG and incubated for a further 18 hours (untolerised controls indicated in bold). NFκB activation is expressed as the mean absorbance units A 620nm ± SD for the CD14 hi/lo M1 and M2 MΦ subsets. Data displayed represents triplicate samples for n = 3 replicate experiments. Significant effects compared to the un-tolerised stimulus control (bold) for each MΦ subset are indicated as *p<0.05, ***p<0.001 and ns, not significant.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques: Incubation, Activation Assay

CD14 lo M1 (a) CD14 hi M1 (b), CD14 lo M2 (c) and CD14 hi M2 (d) MΦ subsets were pre-stimulated (tolerised) with either 100 ng/ml PG-LPS (unshaded) or 1×10 7 cells/ml HKPG (shaded) for 24 hours prior to stimulation with PG-LPS or HKPG and incubated for a further 18 hours (untolerised controls indicated in bold). Pro-inflammatory TNFα cytokine production is expressed in pg/ml as the mean ± SD for the CD14 hi/lo M1 and M2 MΦ subsets. Data displayed represents triplicate samples for n = 3 replicate experiments. Significant effects compared to the un-tolerised stimulus control (bold) for each MΦ subset are indicated as ***p<0.001 and ns, not significant.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: CD14 lo M1 (a) CD14 hi M1 (b), CD14 lo M2 (c) and CD14 hi M2 (d) MΦ subsets were pre-stimulated (tolerised) with either 100 ng/ml PG-LPS (unshaded) or 1×10 7 cells/ml HKPG (shaded) for 24 hours prior to stimulation with PG-LPS or HKPG and incubated for a further 18 hours (untolerised controls indicated in bold). Pro-inflammatory TNFα cytokine production is expressed in pg/ml as the mean ± SD for the CD14 hi/lo M1 and M2 MΦ subsets. Data displayed represents triplicate samples for n = 3 replicate experiments. Significant effects compared to the un-tolerised stimulus control (bold) for each MΦ subset are indicated as ***p<0.001 and ns, not significant.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques: Incubation

CD14 lo M1 (a) CD14 hi M1 (b), CD14 lo M2 (c) and CD14 hi M2 (d) MΦ subsets were pre-stimulated (tolerised) with either 100 ng/ml PG-LPS (unshaded) or 1×10 7 cells/ml HKPG (shaded) for 24 hours prior to stimulation with PG-LPS or HKPG and incubated for a further 18 hours (untolerised controls indicated in bold). Anti-inflammatory IL-10 cytokine production is expressed in pg/ml as the mean ± SD for the CD14 hi/lo M1 and M2 MΦ subsets. Data displayed represents triplicate samples for n = 3 replicate experiments. Significant effects compared to the un-tolerised stimulus controls (bold) for each MΦ subset are are indicated as*p<0.05, **p<0.01, ***p<0.001 and ns, not significant.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: CD14 lo M1 (a) CD14 hi M1 (b), CD14 lo M2 (c) and CD14 hi M2 (d) MΦ subsets were pre-stimulated (tolerised) with either 100 ng/ml PG-LPS (unshaded) or 1×10 7 cells/ml HKPG (shaded) for 24 hours prior to stimulation with PG-LPS or HKPG and incubated for a further 18 hours (untolerised controls indicated in bold). Anti-inflammatory IL-10 cytokine production is expressed in pg/ml as the mean ± SD for the CD14 hi/lo M1 and M2 MΦ subsets. Data displayed represents triplicate samples for n = 3 replicate experiments. Significant effects compared to the un-tolerised stimulus controls (bold) for each MΦ subset are are indicated as*p<0.05, **p<0.01, ***p<0.001 and ns, not significant.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques: Incubation

CD14 hi/lo M1 and M2 MΦ cytokines are differentially tolerised by P.gingivalis and LTA PAMPs.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: CD14 hi/lo M1 and M2 MΦ cytokines are differentially tolerised by P.gingivalis and LTA PAMPs.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques:

Peptidoglycan differentially cross-tolerises P. gingivalis -stimulated CD14 hi/lo M1 and M2 MΦ subsets.

Journal: PLoS ONE

Article Title: Macrophage Subset Sensitivity to Endotoxin Tolerisation by Porphyromonas gingivalis

doi: 10.1371/journal.pone.0067955

Figure Lengend Snippet: Peptidoglycan differentially cross-tolerises P. gingivalis -stimulated CD14 hi/lo M1 and M2 MΦ subsets.

Article Snippet: The THP-1 NFκB reporter cell lines THP-1Blue (CD14 lo ) and THP-1Blue-CD14 (CD14 hi ) (Autogen Bioclear, Calne, UK) were maintained in R10 medium in the presence of the selection antibiotics, zeocin (200 µg/ml) only (CD14 lo ) or 200 µg/ml zeocin and 10 µg/ml blastocidin (CD14 hi ) (Autogen Bioclear, Calne, UK).

Techniques: